# 369: BACILLUS TUSCIAE MEDIUM


## Main sol. 369

4.5	g	Na2HPO4 x 2 H2O	
1.5	g	KH2PO4	
1	g	NH4Cl	
0.01	g	MnSO4 x H2O	
0.2	g	MgSO4 x 7 H2O	
0.01	g	CaCl2 x 2 H2O	
5	mg	Ferric ammonium citrate	
3	ml	**Trace element solution SL-6**	
15	g	Agar, for solid medium	
1000	ml	Distilled water	

1. Adjust pH to 4.0. Agar if necessary 15.0 g. Distribute in 30 - 50 ml amounts in Erlenmeyer flasks and sterilize for 15 minutes at 121°C.
2. Incubate at 50°C without agitation under 5% O2, 10% CO2, 45% H2 (v/v).


## Trace element solution SL-6

0.1	g	ZnSO4 x 7 H2O	
0.03	g	MnCl2 x 4 H2O	
0.3	g	H3BO3	
0.2	g	CoCl2 x 6 H2O	
0.01	g	CuCl2 x 2 H2O	
0.02	g	NiCl2 x 6 H2O	
0.03	g	Na2MoO4 x 2 H2O	
1000	ml	Distilled water