# 204: ASPERGILLUS NIDULANS MINIMAL MEDIUM


## Main sol. 204

500	ml	**Solution A**	
250	ml	**Solution B**	
200	ml	**Solution C**	

1. Autoclave separately for 15 min. Mix A, B and C before pouring plates.


## Solution A

6	g	NaNO3	
1.52	g	KH2PO4	
1.52	g	KCl	
500	ml	Distilled water	

1. Adjust pH to 6.5 with 2 N NaOH.


## Solution B

0.52	g	MgSO4 x 7 H2O	
x		FeSO4 x 7 H2O	
x		ZnSO4 x 7 H2O	
15	g	Agar	
250	ml	Distilled water	


## Solution C

10	g	Glucose	
200	ml	Distilled water