# 1875: Medium MYGS


## Main sol. 1875

1.95	g	MES [2-(N-morpholino) ethane sulfonic acid]	
2	ml	**Trace element solution SL-10**	
2	ml	**Selenite-tungstate solution**	
20	ml	**20 mM MgSO4 x 7 H2O**	
20	ml	**30 mM CaCl2 x 2 H2O**	
20	ml	**20mM (NH4)2HPO4**	
0.5	g	Yeast extract	
3	ml	**Vitamin solution**	
0.4	g	Starch	
0.45	g	D(+)-Glucose	
0.7	ml	Glycerol	
20	g	Agar	
940	ml	Distilled water	

1. Adjust pH to 7.2.
2. Agar und die restlichen Bestandteile getrennt autoklavieren (20 g Agar in 500 ml dest. H2O + Rest der Zutaten in 440 ml dest. H2O)
3. Vitaminlösung erst nach dem Autoklavieren dazugeben


## Trace element solution SL-10

10	ml	HCl (25%)	
1.5	g	FeCl2 x 4 H2O	
70	mg	ZnCl2	
100	mg	MnCl2 x 4 H2O	
6	mg	H3BO3	
190	mg	CoCl2 x 6 H2O	
2	mg	CuCl2 x 2 H2O	
24	mg	NiCl2 x 6 H2O	
36	mg	Na2MoO4 x 2 H2O	
990	ml	Distilled water	

1. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.


## Selenite-tungstate solution

0.5	g	NaOH	
3	mg	Na2SeO3 x 5 H2O	
4	mg	Na2WO4 x 2 H2O	
1000	ml	Distilled water	


## 20 mM MgSO4 x 7 H2O

100	ml	H2O	
0.49	g	MgSO4 x 7 H2O	


## 30 mM CaCl2 x 2 H2O

100	ml	H2O	
0.44	g	CaCl2 x 2 H2O	


## 20mM (NH4)2HPO4

100	ml	H2O	
0.26	g	(NH4)2HPO4	


## Vitamin solution

17	mg	Vitamin B12	
13	mg	4-aminobenzoate	
3	mg	Biotin	
33	mg	Nicotinic acid	
17	mg	Hemicalcium D-(+)-pantothenate	
50	mg	Pyridoxamine hydrochloride	
33	mg	Thiamine-HCl x 2 H2O	
10	mg	DL-6,8-thioctic acid	
10	mg	Riboflavin	
4	mg	Folic acid	
1000	ml	Distilled water	

1. Filter sterilize the solution.