# 1795: PAS (Page’s amoeba saline)


## Main Solution 1795

5	ml	**PAS Stock Solution 1**	
5	ml	**PAS Stock Solution 2**	

1. Make up to <span class='amount' data-volume='1000'>1000</span> ml with MilliQ water.
2. Autoclave at 121 °C for 20 min.


## PAS Stock Solution 1

12	g	NaCl	
0.4	g	MgSO4 x 7 H2O	
0.4	g	CaCl2 x 2 H2O	


## PAS Stock Solution 2

14.2	g	Na2HPO4 (anhydrous)	
13.6	g	KH2PO4 (anhydrous)