# 1602: CY/3-SEAWATER MEDIUM


## Main sol. 1602

20	g	NaCl	
0.45	g	CaCl2 x 2 H2O	
0.33	g	Yeast extract	
1	g	Casitone	
1000	ml	**Sea water salts solution**	
0.5	mg	Cyanocobalamine (vitamin B12)	

1. Adjust pH to 7.5 with 1M NaOH, autoclave at 121°C for 20 min. Add cyanocobalamine from a filter sterilized solution after autoclaving and cooling.


## Sea water salts solution

0.01	g	Ferric citrate	
8	g	MgSO4 x 7 H2O	
1	g	CaCl2 x 2 H2O	
0.5	g	KCl	
0.16	g	NaHCO3	
0.02	g	H3BO3	
0.08	g	KBr	
0.03	g	SrCl2 x 6 H2O	
0.01	g	Sodium beta-glycerophosphate	
1	ml	**Trace element solution SL-4**	
1000	ml	Distilled water	


## Trace element solution SL-4

0.5	g	EDTA	
0.2	g	FeSO4 x 7 H2O	
100	ml	**Trace element solution SL-6**	
900	ml	Distilled water	


## Trace element solution SL-6

0.1	g	ZnSO4 x 7 H2O	
0.03	g	MnCl2 x 4 H2O	
0.3	g	H3BO3	
0.2	g	CoCl2 x 6 H2O	
0.01	g	CuCl2 x 2 H2O	
0.02	g	NiCl2 x 6 H2O	
0.03	g	Na2MoO4 x 2 H2O	
1000	ml	Distilled water