# 1593a: Cyanobacteria Medium BG11+ (TES buffered)


## Main sol. 1593a

2.3	g	TES	
5	ml	NaNO3 (300 g/l stock solution)	
10	ml	Mg(SO4) x 7 H2O (7.5 g/l stock solution)	
10	ml	CaCl2 x 2 H2O (3.6 g/l stock solution)	
10	ml	Na3-citrate x 2 H2O (0.6 g/l stock solution)	
10	ml	Na-EDTA x 2 H2O (0.1 g/l stock solution)	
2	ml	K2HPO4 x 3 H2O (20 g/l stock solution)	
2	ml	Na2CO3 (10 g/l stock solution)	
1	ml	Fe(NH4)citrate (6.0 g/l stock solution)	
1	ml	**Trace metal mix**	

1. Adjust pH to 8.0.
2. Make up to <span class='amount' data-volume='1000'>1000</span> ml with MilliQ water.
3. Autoclave at 121 °C for 20 min.
4. After Cooling, add the following filter sterilized components:

1	ml	Vitamin B12 (0.02 g/l stock solution)	


## Trace metal mix

2.86	g	H3BO3	
1.81	g	MnCl2 x 4 H2O	
0.22	g	ZnSO4 x 7 H2O	
0.39	g	Na2MoO4 x 2 H2O	
10	ml	CuSO4 x 5 H2O (8.0 g/l stock solution)	
10	ml	Co(NO3)2 x 6 H2O (5.0 g/l stock solution)	

1. Make up to <span class='amount' data-volume='1000'>1000</span> ml with MilliQ water.