# 1535: MEDIUM D (20.6%NACL) ## Main sol. 1535 206 g NaCl 0.37 g KCl 36 g MgSO4 x 7 H2O 0.5 g CaCl2 x 2 H2O 0.013 g MnCl2 5 g Yeast extract 20 g Agar 1000 ml Distilled water 1. pH 7.4