# 1511: PLANTOMYCETACEAE MEDIUM SBP2


## Main sol. 1511

0.33	g	KH2PO4	
0.33	g	NH4Cl	
1.2	mg	MgCl2	
1.2	mg	CaCl2	
0.33	g	KCl	
1	ml	**Trace Element Solution**	
1	ml	**Wolin's vitamin solution**	
1000	ml	Distilled water	

1. Prepare the liquid medium. Dispense the medium into culture vessels. Sterilize by autoclaving. Before inoculation add from sterile stock solutions: 

1	g	KNO3	
2	g	Glucose	
1	ml	**Wolin's vitamin solution**	
12.5	ml	NaHCO3 (8% solution)	

2. The 8% NaHCO3 solution may be either filter sterilized or autoclaved. When the solution is autoclaved use tightly closed vessels that are filled to a maximum of 50% of the total volume.
3. The final pH of the medium should be adjusted to pH 7.6-8.0.


## Wolin's vitamin solution

2	mg	Biotin	
2	mg	Folic acid	
10	mg	Pyridoxine hydrochloride	
5	mg	Thiamine HCl	
5	mg	Riboflavin	
5	mg	Nicotinic acid	
5	mg	Calcium D-(+)-pantothenate	
0.1	mg	Vitamin B12	
5	mg	p-Aminobenzoic acid	
5	mg	(DL)-alpha-Lipoic acid	
1000	ml	Distilled water	


## Trace Element Solution

784	mg	Fe(NH4)2(SO4)2 x 6 H2O	
284	mg	NiCl2 x 2 H2O	
38	mg	CoCl2 x 6 H2O	
99	mg	MnCl2 x 4 H2O	
144	mg	ZnSO4 x 7 H2O	
95	mg	Na2SeO4	
33	mg	Na2WO4 x 2 H2O	
24	mg	Na2MoO4 x 2 H2O	
6	mg	H3BO3	
2	mg	CuCl2 x 2 H2O	
1000	ml	Distilled water