# 1421: NATRONORUBRUM THIOOXIDANS MEDIUM


## Main sol. 1421

240	g	NaCl	
3.5	g	K2HPO4	
0.5	g	(NH4)2SO4	
1	ml	**Trace Element Solution (Pfennig & Lippert)**	
1000	ml	Distilled water	

1. Prepare the liquid medium. Adjust to pH 7.5. Before inoculation add from sterile stock solutions: 

16	ml/l	Na-acetate (20 mM)	
6	ml/l	MgSO4 (2mM)	
1	g/l	Yeast extract	


## Trace Element Solution (Pfennig & Lippert)

5	g	EDTA (Trilon B)	
2	g	FeSO4 x 7 H2O	
0.1	g	ZnSO4	
0.03	g	MnCl2	
0.3	g	H3BO3	
0.2	g	CoCl2 x 6 H2O	
0.01	g	CuCl2	
0.02	g	NiCl2 x 2 H2O	
0.02	g	Na2MoO4 x 2 H2O	
1000	ml	Distilled water