# 1308: PY4S


## Main sol. 1308

10	g	Trypticase peptone	
5	g	Yeast extract	
0.25	g	Glucose	
0.25	g	Cellobiose	
0.25	g	Maltose	
0.25	g	Starch (soluble)	
0.5	g	L-Cysteine HCl x H2O	
1	ml	Sodium resazurin (0.1% w/v)	
75	ml	**Solution I**	
75	ml	**Solution II**	
2.5	ml	Na2CO3 (8% w/V)	
10	µg	Vitamin B12	
846.5	ml	Distilled water	

1. The cysteine, Na2CO3, and vitamin B12 are added after the medium has been boiled and cooled under CO2. Adjust pH to 7.3 Distribute under N2 and autoclave.


## Solution I

0.6	g	K2HPO4	
100	ml	Distilled water	


## Solution II

0.12	g	CaCl2 x 2 H2O	
0.12	g	MgSO4 x 7 H2O	
0.6	g	KH2PO4	
1.2	g	(NH4)2SO4	
1.2	g	NaCl	
100	ml	Distilled water