# 1220: ACIDOSOMA MEDIUM


## Main sol. 1220

0.1	g	KH2PO4	
0.25	g	(NH4)2SO4	
0.05	g	CaCl2 x 2 H2O	
0.02	g	MgSO4 x 7 H2O	
1	ml	**Trace elements**	
0.1	g	Yeast extract	
0.5	g	Na gluconate	
1000	ml	Distilled water	
15	g	Agar, for solid medium	

1. Adjust to pH 5.0 - 5.5. Agar may be added at 15 g/l for solid media.


## Trace elements

5	g	EDTA	
0.1	g	CuCl2 x 5 H2O	
2	g	FeSO4 x 7 H2O	
0.1	g	ZnSO4 x 7 H2O	
0.02	g	NiCl2 x 6 H2O	
0.2	g	CoCl2 x 6 H2O	
0.03	g	Na2MoO4	
1000	ml	Distilled water