# 1212: MARINOSPIRILLUM CELERE


## Main sol. 1212

0.5	g	NH4Cl	
0.5	g	KH2PO4	
25	g	NaCl	
0.5	g	Na2SO4	
0.2	g	MgSO4 x 7 H2O	
0.5	g	KCl	
1	g	Yeast extract	
1	g	Peptone	
1	g	Sodium DL-lactate	
15	µg	Vitamin B12	
5	g	Na2CO3	
5	g	NaHCO3	
1	ml	**Trace element solution SL-10 B**	
1000	ml	Distilled water	
15	g	Agar, for solid medium	

1. Autoclave the Na2CO3, NaHCO3 and the lactate separately from the medium and combine after cooling. Vitamin B12 is added from a sterile stock solution. The final pH of the medium should be 9.5. Add 15.0 g/l agar for solid media.


## Trace element solution SL-10 B

1000	ml	Distilled water	
7.7	ml	HCl (25%)	
1.5	g	FeSO4 x 7 H2O	
70	mg	ZnCl2	
100	mg	MnCl2 x 4 H2O	
300	mg	H3BO3	
190	mg	CoCl2 x 6 H2O	
2	mg	CuCl2 x 2 H2O	
24	mg	NiCl2 x 6 H2O	
36	mg	Na2MoO4 x 2 H2O