# 1122a: METHYLOVERSATILIS THERMOTOLERANS MEDIUM


## Main sol. 1122a

2	g	(NH4)2SO4	
2	g	KH2PO4	
0.5	g	NaCl	
0.025	g	MgSO4 x 7 H2O	
2	mg	FeSO4 x 7 H2O	
20	g	Agar (Difco)	
1000	ml	Distilled water	
0.5	%(v/v)	Methanol	
0.1	ml	Vitamin B12 (10µg /ml, filter sterilized)	

1. Adjust to pH 7.2.
2. After autoclaving add methanol (sterilized separately) to a concentration of 0.5 % (v/v) and 0.1 ml vitamin solution B12 (10µg /ml, filter sterilized).