# 1102: MEDIUM FOR ENHYDROMYXA SALSUGINOSA


## Main sol. 1102

0.02	g	Fe(III) citrate	
0.2	g	CaCl2 x 2 H2O	
1.6	g	MgSO4 x 7 H2O	
0.1	g	KCl	
10	g	NaCl	
0.5	g	Na-caseinate	
2	g	HEPES	
0.16	g	NaHCO3	
20	mg	H3BO3	
80	mg	KBr	
30	mg	SrCl2 x 6 H2O	
20	g	Agar	
2	ml	**Trace element solution SL-4**	
998	ml	Distilled water	
0.5	mg	Vitamin B12	

1. Adjust to pH 7.2, check and adjust pH after autoclaving. Add vitamin B12 from a filter sterilized stock solution after autoclaving.


## Trace element solution SL-4

0.5	g	Na2-EDTA	
0.2	g	FeSO4 x 7 H2O	
0.1	g	ZnSO4 x 7 H2O	
0.03	g	MnCl2 x 4 H2O	
0.3	g	H3BO3	
0.2	g	CoCl2 x 6 H2O	
0.01	g	CuCl2 x 2 H2O	
0.02	g	NiCl2 x 6 H2O	
0.03	g	Na2MoO4 x 2 H2O	
1000	ml	Distilled water	

1. First dissolve EDTA in distilled water and adjust pH to 7.0 using 2 N NaOH; then add other compounds.