# 1081a: HORIKOSHI-1 MEDIUM WITH 10% NaCl


## Main sol. 1081a

1000	ml	**Main sol. 1081**	
100	g	NaCl	

1. Use medium 1081 (HORIKOSHI-1 MEDIUM), but add 100 g NaCl.


## Main sol. 1081

10	g	Glucose	
5	g	Polypeptone	
5	g	Yeast extract	
1	g	K2HPO4	
0.2	g	MgSO4 x 7 H2O	
15	g	Agar	
900	ml	Distilled water	
100	ml	Na2CO3 (10 %)	

1. After autoclaving, aseptically add 100 ml of a 10% Na2CO3 solution to the medium. Check final pH to be about 10.0.