# 104d: MALEDIVIBACTER MEDIUM


## Main sol. 104d

5	g	Trypticase peptone (BD BBL)	
5	g	Meat peptone (pepsin-digested)	
10	g	Yeast extract	
40	ml	**Salt solution**	
60	g	NaCl	
5	g	MgSO4 x 7 H2O	
0.5	ml	Sodium resazurin (0.1% w/v)	
6	g	Betaine x H2O	
0.5	g	L-Cysteine HCl x H2O	
960	ml	Distilled water	

1. Dissolve ingredients (except betaine and cysteine), boil medium for 1 min, then cool to room temperature under 100% N2 gas atmosphere. Adjust the pH to 7.2. Thereafter, dispense under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add betaine and cysteine from sterile, anoxic stock solutions prepared under 100% N2. Adjust the pH of the complete medium to 7.3 - 7.5.


## Salt solution

0.25	g	CaCl2 x 2 H2O	
0.5	g	MgSO4 x 7 H2O	
1	g	K2HPO4	
1	g	KH2PO4	
10	g	NaHCO3	
2	g	NaCl	
1000	ml	Distilled water