503: ANAEROBIC FRESHWATER (FWM) MEDIUM

Modifications: Supplement medium after autoclaving with 0.1 g/l yeast extract, 2.0 g/l D-mannose, and 1 ml/l Wolin's vitamin solution (10x) added from anoxic stock solutions sterilized by filtration. Omit D-Glucose.

Growth observed:

Growth conditions: 3-7 days anaerobic 50 °C