Growth observed:
Growth conditions: 3-7 days anaerobic 37 °C
104: PYG MEDIUM (modified)
Modifications: Supplement medium after autoclaving with 50.0 ml/l clarified rumen fluid (see medium 1310), 50.0 ml/l horse serum (Bio & Sell PFD.SE.0500) and 0.2 g/l dithiothreitol (DTT) added from filter-sterilized anoxic stock solutions.
Growth observed:
Growth conditions: 3-7 days anaerobic 37 °C