194: DESULFOBULBUS SP. MEDIUM (FRESHWATER)

Modifications: Na-propionate is replaced with 2.50 g/l Na-L-lactate added after autoclaving from a sterile anoxic stock solution prepared under N2. Supplement medium with 1.00 ml/l seven vitamins solution (see medium 503) added from an anoxic stock solution sterilized by filtration.

Growth observed:

Growth conditions: 2-3 days anaerobic 30 °C