503: ANAEROBIC FRESHWATER (FWM) MEDIUM

Modifications: Supplement medium after autoclaving with 0.6 g/l syringic acid added from a sterile anoxic stock solution neutralized with NaOH. Replace solution D with 1 ml/l Wolin's vitamin solution (10x). Omit D-Glucose.

Growth observed:

Growth conditions: anaerobic 35 °C