925: ALKALIPHILIC SULPHUR RESPIRING STRAINS MEDIUM
Modifications: DSM 13541 = ARh1 (thiosulphate grown) Growth with thiosulfate (40 mM) and NH4Cl (5 mM); in conical flasks 1/10 liquid/air ratio on shaker 200 rpm. Heavy sulfur formation is usual and it is necessary to continue cultivation until the sulfur has been utilized.
Growth observed:
Growth conditions: 7-14 days aerobic 30 °C