Strains for NITROSPIRA MOSCOVIENSIS

Name Growth Modifications External links
Nitrospira defluvii DSM 118082 more than 14 days | 28 °C | aerobic Use 0.02 g/l NaNO2. Use 100 - 150 ml medium in 300 ml Erlenmeyer flasks with metal caps. The medium has to be prepared 2-3 days before inoculation. The final pH should be 7.4-7.6. Inoculate 1 ml per flask and incubate the culture in the dark without stirring. It might take several weeks until the nitrite is consumed. Use a spot test for presence of nitrite on filter paper (e.g., MQuant Nitrite Test, Sigmaldrich 1.10007.0001). DSMZ BacDive
Nitrospira moscoviensis DSM 118083 more than 14 days | 37 °C | aerobic Use 0.02 g/l NaNO2. Use 100 - 150 ml medium in 300 ml Erlenmeyer flasks with metal caps. The medium has to be prepared 2-3 days before inoculation. The final pH should be 7.4-7.6. Inoculate 1 ml per flask and incubate the culture in the dark without stirring. It might take several weeks until the nitrite is consumed. Use a spot test for presence of nitrite on filter paper (e.g., MQuant Nitrite Test, Sigmaldrich 1.10007.0001). DSMZ BacDive
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