Strains for DESULFOTHERMUS MJ MEDIUM (H2/CO2)

Name Growth Modifications External links
Bacterium Desulfothermus okinawensis DSM 17375 50 °C | anaerobic DSMZ BacDive
Hydrogenovibrio sp. DSM 117349 (+) 3-7 days | 20 °C | microaerobic Omit Na-dithionite solution (5% w/v), Yeast extract, Na2S2O3 x 5 H2O, Na-pyruvate, and Na-lactate. Incubate with a gas phase of 75% H2, 15% CO2, and 10% sterile air. Reduce overpressure to 0.5 bar. DSMZ BacDive
Hydrogenovibrio sp. DSM 117350 (+) 3-7 days | 20 °C | microaerobic Omit Na-dithionite solution (5% w/v), Yeast extract, Na2S2O3 x 5 H2O, Na-pyruvate, and Na-lactate. Incubate with a gas phase of 75% H2, 15% CO2, and 10% sterile air. Reduce overpressure to 0.5 bar. DSMZ BacDive
Bacterium Sulfurovum aggregans DSM 27205 2-3 days | 32 °C | microaerobic Supplement medium with 1.00 g/l NaNO3 and omit pyruvate, lactate, yeast extract and dithionite. Upon autoclaving add 10.00 g/l sterile sulfur powder (sterilized by steaming for 3 hours on each of 3 successive days) and adjust pH to 6.0 - 6.5. After inoculation do not pressurize with 80% H2 and 20% CO2 gas mixture and add sterile air in an amount that is equivalent to a volume of 10% of the headspace. DSMZ BacDive
Bacterium Thiofractor thiocamini DSM 22050 2-3 days | 37 °C | anaerobic Supplement medium with 1.00 g/l NaNO3 and omit pyruvate, lactate and yeast extract. Upon autoclaving add 3.00 g/l sterile sulfur powder (sterilized by steaming for 3 hours on each of 3 successive days) and adjust pH to 6.0 - 6.5. DSMZ BacDive
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