Main sol. 1804
Internal Solution-ID: S6304
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Peptone | 0.25 | g | 0.25 | - | |
| Yeast extract | 0.25 | g | 0.25 | - | |
| D-Glucose | 0.25 | g | - | - | |
| Hutner's salts | 20.00 | ml | - | - | |
|
Sea water
(Biomaris MeeresTIEFwasser) |
500.00 | ml | - | - | |
| Distilled water | 500.00 | ml | - | - | |
| 1 Adjust pH to 7.3-7.4. | |||||
| 2 Autoclave at 121 °C for 15 min. | |||||
| 3 After autoclaving, add vitamin solution from a filter sterilized stock solution. | |||||
| Vitamin solution | 10.00 | ml | - | - | |