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0.01% RPA (Rye Grass­-Prescott Agar)

Internal Solution-ID: S6224

This solution is used in 2 media and was first defined in medium C85.

Compound Amount Unit Conc. [g/L] Conc. [mM]
Rye grass
(dried, milled)
0.1 g 0.1 -
Medium C76 1000.0 ml - -
(Oxoid L11)
15.0 g 15 -
1 Make up 1 litre of Rye Grass­Prescott Liquid (RPL -­ see separate recipe), but use only 0.1 g of rye grass. Mix with 15 g of Bacteriological Agar (Oxoid L11). Autoclave at 15 psi for 15 minutes.
C85 RPL/0.01%RPA
C86 RPL:PJ/0.01%RPA