Main sol. 1784
Internal Solution-ID: S5931
Equipment needed: Autoclave
| 1 The medium is a modified MGM which contains 5 g peptone (Oxoid) and 1 g Bacto yeast extract (Difco Laboratories) per liter, and 23% Saltwater (SW) | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Salt Water Solution (30% Stock Solution) | 767 | ml | - | - | |
|
Peptone
(Oxoid) |
5 | g | 5 | - | |
|
Yeast extract
(Difco) |
1 | g | 1 | - | |
| 2 Make up to 1000 ml with distilled water. | |||||
| 3 Adjust pH to 7.2 with Tris-Base (2M). | |||||
| 4 Autoclave at 121 °C for 20 min. | |||||