Main sol. 457f
Internal Solution-ID: S5718
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Medium 457 | 1000.0 | ml | - | - | |
Casamino acids | 0.3 | g/l | 0.3 | - | |
1 For DSM 13022, prepare medium 457 with 0.3 g/l casamino acids. | |||||
2 Prepare a solution of 5 mg/ml phenanthrene in acetone and filter sterilize using a cellulose filter membrane. Add 0.1 ml of this stock solution to the sterilized culture flask. Let acetone evaporate. Add 20 ml medium to the flask. |