Main sol. 1771
Internal Solution-ID: S5509
| 1 pH vor dem Autoklavieren mit NaOH auf 7,2 einstellen; nach dem Autoklavieren getrennt zugeben: | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Sucrose | 103.00 | g | 103 | 300.906 | |
| Glucose | 10.00 | g | 10 | 55.506 | |
| K2SO4 | 0.25 | g | 0.25 | 1.435 | |
| Casamino acids | 0.10 | g | 0.1 | - | |
| Yeast extract | 5.00 | g | 5 | - | |
| TES | 5.73 | g | 5.73 | 24.995 | |
| Trace element solution , welche????? | 2.00 | ml | - | - | |
| Agar | 18.00 | g | 18 | 53.519 | |
| Deionized water | 955.00 | ml | - | - | |
|
CaCl2
(1 M) |
20.00 | ml | - | - | |
|
KH2PO4
(0.54%) |
10.00 | ml | 0.054 | 0.397 | |
|
L-Proline
(20%) |
15.00 | ml | 3 | 26.058 | |