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Vitamin mix (pfennig & Lippert,1966)
Internal Solution-ID: S3031
This solution is used in 2 media and was first defined in medium 1479.
Equipment needed: Filter
| 1 1: acidic solution (HCl, pH3; 100ml) | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Thiamin | 5.0 | mg | 0.013 | 0.047 | |
| Calcium pantothenate | 5.0 | mg | 0.013 | 0.026 | |
| 2 2: basic solution (100ml 0.1N NaOH) | |||||
| Biotin | 2.0 | mg | 0.005 | 0.02 | |
|
PABA
(p-aminobenzoic acid) |
5.0 | mg | 0.013 | 0.091 | |
| Nicotinic acid | 5.0 | mg | 0.013 | 0.102 | |
| Pyridoxine | 10.0 | mg | 0.025 | 0.148 | |
| 3 3: Neutral solution in 100 ml demi water | |||||
| Folic acid | 2.0 | mg | 0.005 | 0.011 | |
| Riboflavin | 5.0 | mg | 0.013 | 0.033 | |
| 4 4: neutral solution in 100 ml demi water | |||||
| B12 | 0.5 | mg | 0.001 | 9.2e-4 | |
| 5 Sterilize by filtration. | |||||
| 6 Mix 1:1:1:1 just after addition or add separatlely, each 1ml/L. | |||||