Main sol. 14
Internal Solution-ID: S19
Equipment needed: Autoclave
Hungate tubes
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Yeast extract | 5.00 | g | 4.946 | - | |
| K2HPO4 | 10.00 | g | 9.891 | 56.789 | |
| Trace element solution SL-4 | 10.00 | ml | - | - | |
|
Pyridoxine hydrochloride
(0.1% w/v) |
1.00 | ml | 9.9e-4 | 0.005 | |
| Na-thioglycolate | 0.75 | g | 0.742 | 6.502 | |
|
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 4.9e-4 | 0.002 | |
| Na2CO3 | 1.00 | g | 0.989 | 9.332 | |
| D-Fructose | 5.00 | g | 4.946 | 27.451 | |
|
Agar
, for solid medium
optional |
15.00 | g | 14.837 | - | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve ingredients (except carbonate and fructose) and sparge medium with 100% N2 gas for at least 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add fructose from a sterile anoxic stock solution prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas atmosphere. Adjust pH of complete medium to 8.0. | |||||
Media with this solution
| 14 | CLOSTRIDIUM FORMICACETICUM MEDIUM |