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Main sol. 12

Internal Solution-ID: S15

Equipment needed: Centrifuge
Compound Amount Unit Conc. [g/L] Conc. [mM]
air-dried garden soil 400 g 400 -
Tap water 1000 ml - -
Agar 15 g 15 44.599
1 Sterilize 400.0 g of air-dried garden soil (with high content of organic matter) in 1000 ml tap water for one hour at 121°C. Allow it to sediment for a few hours at room temperature. Centrifuge the supernatant. Add 15.0 g agar per 1000 ml to the clear supernatant solution thus obtained. Adjust pH to 6.8 - 7.0 and sterilize.