Equipment needed: Hungate tubes
Autoclave
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| K2HPO4 | 0.4 | g | 0.396 | 2.274 | |
| NH4Cl | 1.0 | g | 0.99 | 18.51 | |
| Yeast extract | 2.0 | g | 1.98 | - | |
| Trypticase | 2.0 | g | 1.98 | - | |
| MgCl2 x 6 H2O | 1.0 | g | 0.99 | 4.87 | |
| CaCl2 x 2 H2O | 0.4 | g | 0.396 | 2.694 | |
| Modified Wolin's mineral solution | 10.0 | ml | - | - | |
|
Sodium resazurin
(0.1% w/v) |
0.5 | ml | 5.0e-4 | 0.002 | |
| Na2CO3 | 1.5 | g | 1.485 | 14.012 | |
| Na2S x 9 H2O | 0.3 | g | 0.297 | 1.237 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Dissolve ingredients except carbonate and sulfide, then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add carbonate after autoclaving from an anoxic stock solution prepared under 80% N2 and 20% CO2 gas atmospher and sulfide from a sterile anoxic stock solution autoclaved under 100% N2 gas. Adjust pH of the complete medium to pH 7.0. | |||||
Media with this solution
| 481 | COPROTHERMOBACTER PROTEOLYTICUS MEDIUM |