Main sol. 474
Internal Solution-ID: S974
Equipment needed: Autoclave
| 1 Prepare a suspension of 3.0 g poly-ß-hydroxybutyric acid (PHB) in 100 ml distilled water, stir over night and sonicate until a white homogenous suspension is obtained. Autoclave for 5 minutes at 121°C. Prepare double layer agar plates. First layer: medium 457 with 16.0 g agar per l. | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Medium 457 | 1000.0 | ml | - | - | |
| Agar | 16.0 | g/l | 16 | 47.572 | |
| 2 Second layer: medium 457 with agar, supplemented with 6.6 ml sterile PHB-suspension per 100 ml. | |||||
| Poly-ß-hydroxybutyric acid solution 3%(w/v) | 6.6 | ml | - | - | |
| Medium 457 | 100.0 | ml | - | - | |
Media with this solution
| 474 | MEDIUM WITH POLYHYDROXYBUTYRIC ACID AS CARBON SOURCE |