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Main sol. 465i

Internal Solution-ID: S957

Compound Amount Unit Conc. [g/L] Conc. [mM]
2-chloro-4(ethylamino)-6-(isopropylamino)-1,3,5-triazine , adjust if required
(suspend 10 mg/ml in methanol)
100 mg/l - -
Medium 465 1000 ml - -
1 Suspend atrazine (2-chloro-4(ethylamino)-6-(isopropylamino)-1,3,5-triazine) in methanol (10 mg/ml). Shortly sonicate to reduce particle size. Add stock suspension to medium 465 without nitrogen source (omit ammonium sulphate and calcium nitrate), replace calcium nitrate by calcium chloride) but with 1 g/l glucose as the carbon source. Final concentration of atrazine is 50-100 mg/l.
465i MINERAL MEDIUM WITH ATRAZIN