Equipment needed: Filter
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| K2HPO4 | 0.50 | g | 0.5 | 2.871 | |
| NH4Cl | 1.00 | g | 1 | 18.695 | |
| MgSO4 x 7 H2O | 0.02 | g | 0.02 | 0.081 | |
| Yeast extract | 0.20 | g | 0.2 | - | |
| Casamino acids | 0.10 | g | 0.1 | - | |
| Distilled water | 900.00 | ml | - | - | |
| 1 pH 7.4 | |||||
| 2 Autoclave for 15 min. Add 1 ml of sterile SL-4 (see medium 14) and 100 ml of filter-sterilized phenol-solution (4.7g/l). | |||||
| Trace element solution SL-4 | 1.00 | ml | - | - | |
|
Phenol
(filter-sterilized, 4.7 g/l) |
100.00 | ml | - | - | |
Media with this solution
| 458 | MEDIUM FOR BACILLUS STEAROTHERMOPHILUS |