Back to all solutions

Main sol. 457c

Internal Solution-ID: S918

Equipment needed: Autoclave Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Medium 457 1000 ml - -
3-chloroacrylic acid solution 20 ml - -
1 Prepare medium 457. Make a stock solution of 4 g/l 3-chloroacrylic acid in water, neutralize and filter sterilize. Add 20 ml of this stock solution to 1 l of autoclaved medium. For better growth, 1 g/l glucose may be supplied additionally or instead of the chloroacrylic acid.
457c MEDIUM WITH CHLOROACRYLIC ACID