Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Columbia agar base
(dissolved in 70 % artificial seawater) |
1000.0 | ml | 700 | - | |
| Agar | 2.0 | g/l | 2 | 5.947 | |
| Horse blood OR Sheep blood | 4.0 | % | 40 | - | |
| Glucose | 1.0 | % | 10 | 55.506 | |
| FeCl3 | 2.0 | mM | 0.324 | 2 | |
| Cysteine | 0.1 | % | 1 | 8.254 | |
| 1 Dissolve Columbia agar base in 70% artificial seawater and add additional 2 g agar per liter. Autoclave and mix solution with 4% horse or sheep blood after cooling to 50°C. Add 1% glucose, 2 mM FeCl3 and 0.1% cysteine, mix well and pour plates. Rehydrate pellets of freeze-dried ampoules in liquid medium 220 or 215. | |||||
Media with this solution
| 429d | FRANCISELLA HALIOTICIDA MEDIUM |