Equipment needed: Autoclave
Hungate tubes
Filter
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Potato
(fresh, washed, peeled and sliced) |
200.0 | g | 200 | - | |
| CaCO3 | 2.0 | g | 2 | 19.982 | |
|
Sodium resazurin
(0.1% w/v) |
0.5 | ml | 5.0e-4 | - | |
| D-Glucose | 6.0 | g | 6 | 33.304 | |
| L-Cysteine HCl x H2O | 0.5 | g | 0.5 | 2.847 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Mix potatoes and CaCO3 then autoclave 30 min at 121°C. Filter through cheese cloth, add glucose and cysteine, distribute under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave at 121°C for 20 min. Adjust pH of complete medium to 6.8. | |||||
| 2 Note: For the reactivation of freeze-dried strains it is recommended to use the following simple milk medium: | |||||
Media with this solution
| 411 | CLOSTRIDIUM ACETOBUTYLICUM MEDIUM |