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Main sol. 60

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Internal Solution-ID: S82

Equipment needed: Autoclave Hungate tubes Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 843 ml - -
Solution B 50 ml - -
Solution C 50 ml - -
Solution D 40 ml - -
Solution E 10 ml - -
Solution F 10 ml - -
1 Sparge solution A with 100% CO2 gas for 30 - 45 min to make it anoxic, then distribute under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Autoclave separately solutions B, C, E and F under 100% N2 gas, and solution D under 80% N2 and 20% CO2 gas atmosphere. To complete the medium appropriate amounts of solutions B to F are added to the sterile solution A in the sequence as indicated. Adjust pH of the complete medium to 6.9, if necessary.
60 MOORELLA MEDIUM