Main sol. 394
Internal Solution-ID: S805
Equipment needed: Filter
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Tryptone | 25.0 | g | 23.365 | 328.707 | |
| Yeast extract | 7.5 | g | 7.009 | - | |
|
Tris-HCl
(pH 7.5, 1M) |
20.0 | ml | - | 20 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Autoclave, cool, and then add: | |||||
|
MgCl2
(1M) |
5.0 | ml | 0.476 | 5 | |
|
Diaminopimelic acid
(1%) |
10.0 | ml | 0.1 | 0.526 | |
|
Thymidine
(0.4%) |
10.0 | ml | 0.04 | 0.165 | |
|
Glucose
(20%) |
25.0 | ml | 5 | 27.753 | |
| 2 Magnesium chloride should be sterilized separately by autoclaving, and the other ingredients should be sterilized separately by filtration. | |||||
Media with this solution
| 394 | CHI 1776 MEDIUM |