Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Casein peptone
(tryptic digest) |
10.00 | g | 10 | - | |
| Yeast extract | 5.00 | g | 5 | - | |
| Meat extract | 5.00 | g | 5 | - | |
| Bacto Soytone | 5.00 | g | 5 | - | |
| Glucose | 10.00 | g | 10 | 55.506 | |
| K2HPO4 | 2.00 | g | 2 | 11.483 | |
| MgSO4 x 7 H2O | 0.20 | g | 0.2 | 0.811 | |
| MnSO4 x H2O | 0.05 | g | 0.05 | 0.296 | |
| Tween 80 | 1.00 | ml | - | - | |
| NaCl | 5.00 | g | 5 | 85.558 | |
| L-Cysteine HCl x H2O | 0.50 | g | 0.5 | - | |
| Salt solution | 40.00 | ml | - | - | |
|
Resazurin
(25 mg/100ml) |
4.00 | ml | - | - | |
| Distilled water | 950.00 | ml | - | - | |
| 1 The cysteine are added after the medium has been boiled and cooled under CO2. Adjust pH to 6.8 using 8 N NaOH. Distribute under N2 and autoclave. | |||||
Media with this solution
| 58 | BIFIDOBACTERIUM MEDIUM |