Equipment needed: Autoclave
Hungate tubes
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| KH2PO4 | 0.50 | g | 0.499 | 3.663 | |
| MgSO4 x 7 H2O | 0.40 | g | 0.399 | 1.618 | |
| NaCl | 0.40 | g | 0.399 | 6.824 | |
| NH4Cl | 0.25 | g | 0.249 | 4.66 | |
| CaCl2 x 2 H2O | 0.05 | g | 0.05 | 0.339 | |
| Trace element solution SL-10 | 1.00 | ml | - | - | |
| Selenite-tungstate solution | 1.00 | ml | - | - | |
| Na-acetate | 1.00 | g | 0.997 | 12.154 | |
|
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 5.0e-4 | 0.002 | |
| Na2CO3 | 1.00 | g | 0.997 | 9.407 | |
|
Isopropyl alcohol
(25% v/v) |
30.00 | ml | - | - | |
| Na2S x 9 H2O | 0.50 | g | 0.499 | 2.076 | |
| Distilled water | 970.00 | ml | - | - | |
| 1 Dissolve ingredients except carbonate, isopropyl alcohol and sulfide. Sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Add and dissolve bicarbonate, then dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add isopropyl alcohol and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. Prior to use check pH of complete medium and adjust to 7.0 - 7.2, if necessary. | |||||
Media with this solution
| 374 | METHANOSPIRILLUM SK MEDIUM |