Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Linsmaier & Skoog Med. incl. vitamins
(Duchefa L0230) |
4.400 | g | 4.4 | - | |
| 2,4-Dichlorophenoxyacetic Acid Stock Solution | 0.220 | ml | - | - | |
| 1-Naphtylacetic Acid Stock Solution | 0.186 | ml | - | - | |
|
Sucrose
(Duchefa S0809) |
30.000 | g | 30 | 87.642 | |
| 1 Make up to 1000 ml with MilliQ water. | |||||
| 2 Adjust pH to 6.0. | |||||
| 3 For solid medium add Plant Agar as followed: | |||||
|
Plant Agar
(Duchefa P1001) |
9.000 | g | 9 | - | |
| 4 Autoclave at 121 °C for 20 min. | |||||
Media with this solution
| 1884 | Linsmaier & Skoog LS Plant Cell Medium |