Equipment needed: Autoclave
sterile filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Peptone | 5 | g | - | - | |
| Meat extract | 3 | g | - | - | |
| Agar , for solid medium | 15 | g | - | - | |
| Distilled water | 1000 | ml | - | - | |
| Bromothymol blue | 25 | mg | - | - | |
| 2,3,5-triphenyltetrazolium chloride stock solution | 4 | ml | - | - | |
| 1 Autoclave at 121 °C for 21 min. | |||||
| 2 After autoclavation add 4 mL from a sterile 2,3,5-triphenyltetrazolium chloride stock solution (1% w/v). | |||||
Media with this solution
| 1c | Nutrient bromothymol blue agar NBTA |