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Main sol. 1875

Internal Solution-ID: S6566

Compound Amount Unit Conc. [g/L] Conc. [mM]
MES [2-(N-morpholino) ethane sulfonic acid] 1.95 g 1.95 9.988
Trace element solution SL-10 2.00 ml - -
Selenite-tungstate solution 2.00 ml - -
20 mM MgSO4 x 7 H2O 20.00 ml - -
30 mM CaCl2 x 2 H2O 20.00 ml - -
20mM (NH4)2HPO4 20.00 ml - -
Yeast extract 0.50 g 0.5 -
Vitamin solution 3.00 ml - -
Starch 0.40 g 0.4 -
D(+)-Glucose 0.45 g 0.45 2.498
Glycerol 0.70 ml - -
Agar 20.00 g 20 -
Distilled water 940.00 ml - -
1 Adjust pH to 7.2.
2 Agar und die restlichen Bestandteile getrennt autoklavieren (20 g Agar in 500 ml dest. H2O + Rest der Zutaten in 440 ml dest. H2O)
3 Vitaminlösung erst nach dem Autoklavieren dazugeben
1875 Medium MYGS