Main sol. 340
Internal Solution-ID: S653
Equipment needed: Gassing station
Hungate tubes
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Trypticase
(BBL) |
17 | g | 17 | - | |
| Yeast extract | 3 | g | 3 | - | |
| Glucose | 3 | g | 3 | 16.652 | |
| NaCl | 3 | g | 3 | 51.335 | |
| KNO3 | 3 | g | 3 | 29.673 | |
| Haemin | 3 | mg | 0.003 | 0.005 | |
| Distilled water | 1000 | ml | - | - | |
| 1 Adjust pH to 7.0. | |||||
| 2 Sterilize glucose separately. Incubate in oxygen-free, 5 - 10% CO2 containing atmosphere. | |||||
Media with this solution
| 340 | CAPNOCYTOPHAGA MEDIUM |