Equipment needed: Autoclave
Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 26.0 | g | 26 | 444.901 | |
| MgSO4 x 7 H2O | 5.0 | g | 5 | 20.286 | |
| MgCl2 x 6 H2O | 5.0 | g | 5 | 24.594 | |
| CaCl2 x 2 H2O | 1.5 | g | 1.5 | 10.203 | |
| KBr | 0.1 | g | 0.1 | 0.84 | |
| NaHCO3 buffer (1M) | 2.0 | ml | - | - | |
| HEPES buffer solution | 10.0 | ml | - | - | |
| KH2PO4 solution (2.93 nM) | 5.0 | ml | - | - | |
| Trace element solution (Nitrososphaera) | 1.0 | ml | - | - | |
| FeNaEDTA solution (7.5 mM) | 1.0 | ml | - | - | |
| NH4Cl (1 M) | 2.0 | ml | - | - | |
| Double distilled water | 1000.0 | ml | - | - | |
| 1 Incubate all used glass ware and magnetic stirring rods in 0.1 M HCl and rinse 3x with double distilled water to remove residual detergents (always check visual for soap bubbles). | |||||
| 2 Autoclave the basic salt water medium and cool to room temperature. This medium should be free of any precipitations! | |||||
| 3 Aseptically, add per L of basic salt water medium the following solutions from sterile stocks (do not store complemented SCM): Trace element solution (Nitrososphaera), FeNaEDTA solution, NaHCO3 solution, HEPES buffer solution, NH4Cl solution, KH2PO4 solution | |||||
| 4 Adjust pH to 7.6. | |||||
Media with this solution
| 1630e | Synthetic Crenarchaeota Medium (SCM) |