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Main sol. 1801

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Internal Solution-ID: S6287

Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 1001 ml - -
Solution B 15 ml - -
Solution C 2 ml - -
Solution D 2 ml - -
Solution E 2 ml - -
Solution F 3 ml - -
Solution G 1 ml - -
1 Dissolve ingredients of solution A (except MnO2), then sparge with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Distribute solution A under the same gas atmosphere into anoxic Hungate-type tubes or serum vials containing the appropriate amount of MnO2 and autoclave at 121°C for 15 min. Solution B is autoclaved separately under an 80% N2 and 20% CO2 gas atmosphere, and solutions C, D, and E are autoclaved under a 100% N2 gas atmosphere. Solutions F and G are prepared under 100% N2 gas atmosphere and sterilized by filtration. To complete the medium appropriate amounts of solutions B to G are added to the sterile solution A in the sequence as indicated. The final pH of the complete medium should be 7.5.
1801 SULFURIMONAS MARISNIGRI MEDIUM