Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| TES | 2.3 | g | 2.3 | 10.033 | |
|
NaNO3
(300 g/l stock solution) |
5.0 | ml | - | - | |
|
Mg(SO4) x 7 H2O
(7.5 g/l stock solution) |
10.0 | ml | - | - | |
|
CaCl2 x 2 H2O
(3.6 g/l stock solution) |
10.0 | ml | - | - | |
|
Na3-citrate x 2 H2O
(0.6 g/l stock solution) |
10.0 | ml | - | - | |
|
Na-EDTA x 2 H2O
(0.1 g/l stock solution) |
10.0 | ml | - | - | |
|
K2HPO4 x 3 H2O
(20 g/l stock solution) |
2.0 | ml | - | - | |
|
Na2CO3
(10 g/l stock solution) |
2.0 | ml | - | - | |
|
Fe(NH4)citrate
(6.0 g/l stock solution) |
1.0 | ml | - | - | |
| Trace metal mix | 1.0 | ml | - | - | |
| 1 Adjust pH to 8.0. | |||||
| 2 Make up to 1000 ml with MilliQ water. | |||||
| 3 Autoclave at 121 °C for 20 min. | |||||
| 4 After Cooling, add the following filter sterilized components: | |||||
|
Vitamin B12
(0.02 g/l stock solution) |
1.0 | ml | - | - | |
Media with this solution
| 1593a | Cyanobacteria Medium BG11+ (TES buffered) |