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Mail sol. C34
Internal Solution-ID: S6025
This solution is used in 2 media and was first defined in medium C34.
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
NaNO3
(15 g/200 ml stock solution) |
1.0 | ml | - | - | |
|
NaH2PO4 x 2 H2O
(1.13 g/200 ml stock solution) |
1.0 | ml | - | - | |
| Trace elements (x10 concentration) | 0.1 | ml | - | - | |
| Vitamin mix | 1.0 | ml | - | - | |
|
Natural sea water
(filtered) |
1000.0 | ml | - | - | |
| Agar , for solid medium | 15.0 | g | 15 | - | |
| 1 Make up to 1 litre with filtered natural seawater. Adjust pH to 8.0 with 1M NaOH or 1 M HCl prior to autoclaving. For agar add 15g per litre Bacteriological Agar. Autoclave at 15 psi for 15 minutes. | |||||